Raw Data for BglB Variant R246K

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of R246K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		1.18E-03	2.06E-03
	B	48.7	1.56E-05	4.99E-03	3.07E-03
	C	46.2	3.25E-03	3.45E-03	2.61E-03
	D	42.3	4.75E-03	4.73E-03
	E	37.7	2.06E-02	2.19E-02	2.23E-02
	F	33.9	5.11E-02	5.13E-02	5.50E-02
	G	31.4	5.74E-02	5.63E-02	5.80E-02
	H	30.0	5.32E-02	4.76E-02	5.71E-02

Download these data

Protein purified on 4/5/2024 and assayed on 4/12/2024.
Data uploaded by Michellexu and last updated on 2024-04-12 12:46:21.

Reference Data for WT

slope of R246K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.11E-03	2.68E-03	5.21E-03
	B	48.7	2.47E-03	2.66E-03
	C	46.2	3.72E-03		4.16E-03
	D	42.3	2.92E-02	3.06E-02	2.96E-02
	E	37.7	9.53E-02	9.60E-02	9.52E-02
	F	33.9	9.98E-02	9.73E-02	7.86E-02
	G	31.4	8.40E-02	7.76E-02	7.24E-02
	H	30.0	9.00E-02		8.90E-02

Download these data

Protein purified on 4/5/2024 and assayed on 4/12/2024.
Data uploaded by afeuer and last updated on 2024-04-12 12:37:27.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.