D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R297A

Expression Data

Yield (mg/mL): 0.183

Kinetic Assay Data

**slope of R297A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.16E-03	3.34E-03	3.24E-03
B	25.00	2.72E-03	3.23E-03	2.74E-03
C	8.33	0.002080882	0.002144118	0.002204412
D	2.78	0.000885294	0.000914706	0.000905882
E	0.93	0.000372059	0.000395588	0.000382353
F	0.31
G	0.10
H	0.03

Yield (mg/mL): 0.183 Dilution Factor: 10×

Protein purified on 4/9/2024 and assayed on 4/9/2024.
Data uploaded by RyeRye and last updated on 2024-04-22 09:47:56.

Thermostability Assay Data

**slope of R297A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	1.63E-03	1.34E-03	1.73E-03
B	31.3	1.55E-03	1.01E-03	2.01E-03
C	33.6	1.64E-03	1.73E-03	1.64E-03
D	37.7		6.03E-04	6.59E-04
E	42.4	4.35E-04	3.76E-04	4.87E-04
F	45.7	0		0
G	48.3	0	0	0
H	50.0	1.01E-04	0	1.85E-04

Protein purified on 4/9/2024 and assayed on 4/9/2024.
Data uploaded by RyeRye and last updated on 2024-04-22 09:34:55.

Reference Data for WT

**slope of R297A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	30.0	1.32E-02	1.16E-02	1.25E-02
B	31.3	1.20E-02	1.27E-02	1.03E-02
C	33.6	1.16E-02	1.22E-02	9.44E-03
D	37.7	6.30E-03	5.29E-03	6.89E-03
E	42.4		6.20E-03	6.62E-03
F	45.7	3.62E-04	5.14E-05	2.25E-04
G	48.3	1.38E-04	9.85E-05	1.28E-04
H	50.0	2.34E-04	1.66E-04	4.26E-05

Protein purified on 4/9/2024 and assayed on 4/11/2024.
Data uploaded by jacksonjones and last updated on 2024-04-22 21:31:52.

Design Data

Rosetta Score for R297A: 5.914

Citations

The unpublished data above were collected by students from University of Alabama, Huntsville. For inquiries, please contact Sharifa: <str0012@uah.edu>.