D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L171F

Expression Data

Yield (mg/mL): 8.121

Kinetic Assay Data

**slope of L171F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.8425
B	25.00	0.6625	0.698	0.7245
C	8.33	0.506	0.5215	0.561
D	2.78	0.2905	0.327	0.2595
E	0.93	0.127	0.1295	0.0935
F	0.31	0.0455	0.046	0.0355
G	0.10	0.016	0.017	0.0125
H	0.03	0.0005	0.0005	0.0005

Yield (mg/mL): 8.121 Dilution Factor: 100×

Protein purified on 4/4/24 and assayed on 4/11/24.
Data uploaded by cyazji and last updated on 2024-04-18 12:14:46.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6405		0.615
B	25.00	0.5205	0.493	0.543
C	8.33	0.439	0.441	0.396
D	2.78	0.2265	0.2275	0.237
E	0.93	0.099	0.1	0.1005
F	0.31	0.0405	0.035	0.037
G	0.10	0.012	0.013	0.013
H	0.03	1.48E-16	1.48E-16

Yield (mg/mL): 6.194 Dilution Factor: 100×

Protein purified on 4/4/24 and assayed on 4/11/24.
Data uploaded by cyazji and last updated on 2024-04-18 11:27:29.

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.