Raw Data for BglB Variant W399F
Expression Data
0.581
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 6.67E-05 | 6.78E-05 | |||
| B | 25.00 | |||||
| C | 8.33 | 3.39E-05 | 3.39E-05 | |||
| D | 2.78 | |||||
| E | 0.93 | 1.70E-05 | 1.67E-05 | 1.67E-05 | ||
| F | 0.31 | |||||
| G | 0.10 | 1.67E-05 | 1.67E-05 | |||
| H | 0.03 | 1.67E-05 | 1.67E-05 | 1.67E-05 |
Protein purified on 4/10/2024 and assayed on 4/11/2024.
Data uploaded by NicoleStark and last updated on 2024-04-23 14:29:19.
Reference Data for WT
Wild-type data not found. It may have been rejected in the curation process and deleted from the database.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 30.0 | 3.90E-06 | 2.13E-05 | |||
| B | 33.6 | 4.66E-06 | 1.62E-05 | |||
| C | 37.3 | 4.19E-06 | 8.55E-06 | |||
| D | 39.1 | 4.56E-06 | 1.15E-05 | |||
| E | 40.9 | 2.79E-06 | 9.12E-06 | |||
| F | 44.5 | 1.81E-06 | 5.68E-06 | |||
| G | 48.2 | 4.46E-06 | 2.13E-06 | |||
| H | 50.0 | 2.87E-06 |
Reference Data for WT
Wild-type data not found. It may have been rejected in the curation process and deleted from the database.
Design Data
Citations
The unpublished data above were collected by students from University of Alabama, Huntsville. For inquiries, please contact Emily Hunt: <ech0012@uah.edu>.