Raw Data for BglB Variant W409H

Expression Data

SDS-PAGE
 0.5785

Kinetic Assay Data

slope of W409H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1087 0.111 0.1042
B 25.00 0.0973 0.0962 0.09275
C 8.33 0.07841 0.05555 0.07785
D 2.78 0.05695 0.0556 0.05615
E 0.93 0.0335 0.0317 0.03275
F 0.31 0.01485 0.01375 0.01420
G 0.10 0.00535 0.0053 0.005250
H 0.03 0 0 0
 0.5785      100×

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by alexpro and last updated on 2024-05-15 11:16:07.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3376 0.3436 0.3504
B 25.00 0.2532 0.2706 0.2968
C 8.33 0.1996 0.22 0.2298
D 2.78 0.1239 0.1329 0.0986
E 0.93 0.05435 0.05345 0.05355
F 0.31 0.02135 0.02225 0.0214
G 0.10 0.00755 0.008451 0.00735
H 0.03 0 0 0
 0.849      100×

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by alexpro and last updated on 2024-06-07 13:40:07.


Thermostability Assay Data

slope of W409H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0005000 0.0003500 0.0005500
C 45.7 0.001250 0.001150
D 42.4 0.02555 0.02175 0.01785
E 37.7 0.07700 0.06235 0.08200
F 33.6 0.1000 0.08985
G 31.3 0.1229 0.1213 0.09370
H 30.0 0.1029 0.1139 0.09950

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by alexpro and last updated on 2024-05-15 11:42:42.


Reference Data for WT

slope of W409H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0003500 0.0003500
B 48.3 0.0007000 0.0008001 0.0005000
C 45.7 0.001000 0.0008500 0.0009500
D 42.4 0.02685 0.02310 0.02705
E 37.7 0.1322 0.1526 0.1571
F 33.6 0.1433 0.1593 0.1555
G 31.3 0.1289 0.1240 0.1301
H 30.0 0.1135 0.1447 0.1833

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by alexpro and last updated on 2024-05-15 11:30:08.


Design Data

2.151

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.