D2D CURE | Data | Raw Characterization Data

Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.849

Kinetic Assay Data

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3376	0.3436	0.3504
B	25.00	0.2532	0.2706	0.2968
C	8.33	0.1996	0.22	0.2298
D	2.78	0.1239	0.1329	0.0986
E	0.93	0.05435	0.05345	0.05355
F	0.31	0.02135	0.02225	0.0214
G	0.10	0.00755	0.008451	0.00735
H	0.03	0	0	0

Yield (mg/mL): 0.849 Dilution Factor: 100×

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by alexpro and last updated on 2024-06-07 13:40:07.

Thermostability Assay Data

**slope of WT A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.0003500	0.0003500
B	48.3	0.0007000	0.0008001	0.0005000
C	45.7	0.001000	0.0008500	0.0009500
D	42.4	0.02685	0.02310	0.02705
E	37.7	0.1322	0.1526	0.1571
F	33.6	0.1433	0.1593	0.1555
G	31.3	0.1289	0.1240	0.1301
H	30.0	0.1135	0.1447	0.1833

Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by alexpro and last updated on 2024-05-15 11:30:08.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.