Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.862

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.33E-01	2.16E-01	2.26E-01
	B	25.00	1.74E-01	1.77E-01	1.82E-01
	C	8.33	1.36E-01	1.29E-01	1.28E-01
	D	2.78	8.24E-02	7.99E-02	7.69E-02
	E	0.93	3.63E-02	3.55E-02	3.60E-02
	F	0.31	1.43E-02	1.52E-02	1.34E-02
	G	0.10	5.00E-03	5.15E-03	5.00E-03
	H	0.03		0	0

Yield (mg/mL): 0.862     Dilution Factor:  100×

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Protein purified on 4/10/2024 and assayed on 4/17/2024.
Data uploaded by YvonneP and last updated on 2024-05-15 11:33:33.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	3.50E-04	2.50E-04	2.00E-04
	B	48.3	2.50E-04		2.50E-04
	C	45.7	5.50E-04	3.50E-04	5.00E-04
	D	42.4	1.91E-02	2.00E-02	2.31E-02
	E	37.7	1.96E-01	1.62E-01	9.21E-02
	F	33.6		1.70E-01	1.67E-01
	G	31.3	1.75E-01	1.81E-01	2.10E-01
	H	30.0	1.96E-01	1.84E-01	1.81E-01

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Protein purified on 4/10/24 and assayed on 4/17/24.
Data uploaded by ejaneckova and last updated on 2024-05-16 17:47:18.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.