D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L108A

Expression Data

Yield (mg/mL): 1.166

Kinetic Assay Data

**slope of L108A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.26E-01	2.07E-01	2.30E-01
B	25.00	2.35E-01	2.04E-01	1.91E-01
C	8.33	1.65E-01	1.98E-01	1.75E-01
D	2.78	1.25E-01	1.23E-01	1.22E-01
E	0.93	7.98E-02	7.22E-02	7.68E-02
F	0.31	2.63E-02	2.34E-02	2.99E-02
G	0.10	1.58E-02	1.73E-02	1.73E-02
H	0.03	8.00E-04	5.50E-04	1.10E-03

Yield (mg/mL): 1.166 Dilution Factor: 10×

Protein purified on 2024/2/20 and assayed on 2024/2/22.
Data uploaded by YuanAngela666 and last updated on 2024-05-22 16:10:25.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.35E-01	2.33E-01	2.28E-01
B	25.00	2.19E-01	2.21E-01	1.97E-01
C	8.33	1.72E-01	1.73E-01	1.71E-01
D	2.78	1.08E-01	1.10E-01	1.04E-01
E	0.93	5.11E-02	5.26E-02	5.87E-02
F	0.31	1.65E-02	1.93E-02	1.80E-02
G	0.10	1.07E-02	1.33E-02	1.25E-02
H	0.03	4.50E-04	4.00E-04	5.50E-04

Yield (mg/mL): 3.508 Dilution Factor: 100×

Protein purified on 2024/2/20 and assayed on 2024/2/22.
Data uploaded by YuanAngela666 and last updated on 2024-05-15 14:27:50.

Design Data

Rosetta Score for L108A: -0.652

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.