Raw Data for BglB Variant W409F

Expression Data

SDS-PAGE
 0.454

Kinetic Assay Data

slope of W409F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.07096 0.08155 0.08096
B 25.00 0.0705 0.07255 0.07016
C 8.33 0.05385 0.05465 0.058
D 2.78 0.03915 0.0349 0.035
E 0.93 0.01895 0.02065 0.0201
F 0.31 0.008201 0.0088 0.00815
G 0.10 0.0031 0.00305 0.0032
H 0.03 0.0002 0 0.0003
 0.454      100×

Protein purified on 4/11/24 and assayed on 4/18/24.
Data uploaded by SashaG and last updated on 2024-05-16 11:26:33.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.68E-01 1.79E-01 1.78E-01
B 25.00 1.40E-01 1.56E-01 1.45E-01
C 8.33 1.12E-01 1.13E-01 1.11E-01
D 2.78 7.05E-02 6.77E-02 6.34E-02
E 0.93 3.37E-02 2.96E-02 2.94E-02
F 0.31 1.14E-02 1.16E-02 1.24E-02
G 0.10 4.05E-03 4.35E-03
H 0.03 2.50E-04 2.00E-04 1.50E-04
 0.901      100×

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by mheisler and last updated on 2024-05-16 11:34:38.


Thermostability Assay Data

slope of W409F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00 0.00E+00
B 48.3 0.00E+00 0.00E+00
C 45.7 0.00E+00 5.00E-04 1.50E-03
D 42.7 1.35E-02 1.15E-02 9.50E-03
E 37.7 6.52E-02 6.41E-02 6.48E-02
F 33.6 7.73E-02 7.94E-02 8.00E-02
G 31.3 7.82E-02 7.98E-02 8.00E-02
H 30.0 8.97E-02 9.11E-02 8.38E-02

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by SamN and last updated on 2024-05-16 11:38:29.


Reference Data for WT

slope of W409F A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 0.00E+00 4.00E-04
C 45.7 5.00E-04 6.00E-04
D 42.7 2.54E-02 2.52E-02 3.18E-02
E 37.7 1.61E-01 1.63E-01 1.57E-01
F 33.6 1.91E-01 1.75E-01 1.74E-01
G 31.3 1.74E-01 1.78E-01 1.79E-01
H 30.0 1.84E-01 1.79E-01 1.81E-01

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by SamN and last updated on 2024-05-16 11:40:46.


Design Data

1.197

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.