Raw Data for BglB Variant W409M

Expression Data

SDS-PAGE
 0.623

Kinetic Assay Data

slope of W409M A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 6.40E-03 6.25E-03 6.50E-03
B 25.00 4.85E-03 4.50E-03 4.35E-03
C 8.33 3.60E-03 3.55E-03 3.20E-03
D 2.78
E 0.93 1.90E-03 2.00E-03
F 0.31
G 0.10 0.00E+00 0.00E+00
H 0.03
 0.623      100×

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by KevinT and last updated on 2024-05-16 11:34:25.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.68E-01 1.79E-01 1.78E-01
B 25.00 1.40E-01 1.56E-01 1.45E-01
C 8.33 1.12E-01 1.13E-01 1.11E-01
D 2.78 7.05E-02 6.77E-02 6.34E-02
E 0.93 3.37E-02 2.96E-02 2.94E-02
F 0.31 1.14E-02 1.16E-02 1.24E-02
G 0.10 4.05E-03 4.35E-03
H 0.03 2.50E-04 2.00E-04 1.50E-04
 0.901      100×

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by mheisler and last updated on 2024-05-16 11:34:38.


Thermostability Assay Data

slope of W409M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 0.00E+00 9.00E-04
B 48.3 0.00E+00 0.00E+00
C 45.7 7.00E-04 6.00E-04 4.00E-04
D 42.4 2.04E-02 1.80E-02 1.72E-02
E 37.7 9.44E-02 9.40E-02 9.65E-02
F 33.6 1.03E-01 1.09E-01 1.08E-01
G 31.3 1.12E-01 1.14E-01 1.11E-01
H 30.0 1.12E-01 1.14E-01 1.12E-01

Protein purified on 4/18/2024 and assayed on 4/11/2024.
Data uploaded by natashaloza and last updated on 2024-05-16 11:41:45.


Reference Data for WT

slope of W409M A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00
B 48.3 0.00E+00 4.00E-04
C 45.7 5.00E-04 6.00E-04
D 42.7 2.54E-02 2.52E-02 3.18E-02
E 37.7 1.61E-01 1.63E-01 1.57E-01
F 33.6 1.91E-01 1.75E-01 1.74E-01
G 31.3 1.74E-01 1.78E-01 1.79E-01
H 30.0 1.84E-01 1.79E-01 1.81E-01

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by SamN and last updated on 2024-05-16 11:40:46.


Design Data

-0.639

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.