Raw Data for BglB Variant W409A
Expression Data
0.448
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 2.34E-02 | 1.82E-02 | 2.34E-02 | ||
| B | 25.00 | 1.78E-02 | 1.76E-02 | 1.77E-02 | ||
| C | 8.33 | 9.70E-03 | 1.07E-02 | |||
| D | 2.78 | 9.70E-03 | 7.70E-03 | 6.50E-03 | ||
| E | 0.93 | 5.30E-03 | 4.40E-03 | 3.50E-03 | ||
| F | 0.31 | 2.10E-03 | 1.90E-03 | 1.50E-03 | ||
| G | 0.10 | 9.00E-04 | 1.00E-03 | 8.00E-04 | ||
| H | 0.03 | 0.00E+00 | 4.90E-04 | 1.80E-03 |
Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by DeniseSoto and last updated on 2024-05-16 11:38:04.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.18E-01 | 1.17E-01 | 1.10E-01 | ||
| B | 25.00 | 1.01E-01 | 8.86E-02 | 8.72E-02 | ||
| C | 8.33 | 6.06E-02 | 5.76E-02 | 4.79E-02 | ||
| D | 2.78 | 2.94E-02 | 2.96E-02 | 2.81E-02 | ||
| E | 0.93 | 1.13E-02 | 1.09E-02 | 1.09E-02 | ||
| F | 0.31 | 4.00E-03 | 3.90E-03 | 3.60E-03 | ||
| G | 0.10 | 1.50E-03 | 1.60E-03 | 1.50E-03 | ||
| H | 0.03 | 0.00E+00 | 5.00E-04 | 8.00E-04 |
Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by KeairaC and last updated on 2024-05-16 11:30:43.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 5.00E-04 | 4.00E-04 | 6.00E-04 | ||
| B | 48.3 | 8.00E-04 | 0.00E+00 | 5.00E-04 | ||
| C | 45.7 | 6.00E-04 | 7.00E-04 | 5.00E-04 | ||
| D | 42.4 | 7.20E-03 | 8.30E-03 | 8.60E-03 | ||
| E | 37.7 | 3.55E-02 | 3.88E-02 | 3.48E-02 | ||
| F | 33.6 | 3.69E-02 | 3.82E-02 | |||
| G | 31.3 | 4.37E-02 | 4.38E-02 | 4.39E-02 | ||
| H | 30.0 | 4.26E-02 | 4.40E-02 | 4.20E-02 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 5.00E-04 | 6.00E-04 | |||
| B | 48.3 | 3.00E-04 | 0.00E+00 | 4.00E-04 | ||
| C | 45.7 | 5.00E-04 | 6.00E-04 | 7.00E-04 | ||
| D | 42.4 | 2.66E-02 | 2.65E-02 | 3.02E-02 | ||
| E | 37.7 | 1.36E-01 | 1.31E-01 | 1.31E-01 | ||
| F | 33.6 | 1.40E-01 | 1.36E-01 | |||
| G | 31.3 | 1.38E-01 | 1.35E-01 | 1.34E-01 | ||
| H | 30.0 | 1.44E-01 | 1.44E-01 |
Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by KeairaC and last updated on 2024-05-16 11:40:39.
Design Data
Citations
The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.