Raw Data for BglB Variant W409K

Expression Data

Yield (mg/mL): 0.478

Kinetic Assay Data

slope of W409K A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.68E-02	2.94E-02	2.59E-02
	B	25.00	1.93E-02	2.28E-02	2.42E-02
	C	8.33	2.35E-02	1.97E-02	1.97E-02
	D	2.78	1.49E-02	1.64E-02	1.50E-02
	E	0.93	9.85E-03	1.10E-02	9.10E-03
	F	0.31	4.60E-03	5.80E-03	4.50E-03
	G	0.10	2.20E-03	1.65E-03	1.85E-03
	H	0.03	3.00E-04		3.00E-04

Yield (mg/mL): 0.478     Dilution Factor:  100×

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Protein purified on 4/11/24 and assayed on 4/18/24.
Data uploaded by nponce and last updated on 2024-05-16 11:40:19.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.55E-01	1.48E-01	1.52E-01
	B	25.00	1.12E-01	1.12E-01	1.13E-01
	C	8.33	9.35E-02	8.09E-02	8.92E-02
	D	2.78	5.02E-02	4.68E-02	4.17E-02
	E	0.93	2.01E-02	2.11E-02
	F	0.31	9.10E-03	8.90E-03	9.40E-03
	G	0.10	3.85E-03	3.65E-03	3.65E-03
	H	0.03		4.50E-04	4.00E-04

Yield (mg/mL): 1.057     Dilution Factor:  100×

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Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by JustinR and last updated on 2024-05-16 11:49:40.

Thermostability Assay Data

slope of W409K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.00E+00	0.00E+00
	B	48.3	5.00E-04		5.00E-04
	C	45.7	9.00E-04	4.00E-04	7.00E-04
	D	42.4	6.60E-03	7.00E-03	5.80E-03
	E	37.7		2.80E-02	2.71E-02
	F	33.6	3.54E-02	3.55E-02	3.40E-02
	G	31.3	4.48E-02	4.24E-02	3.86E-02
	H	30.0	4.01E-02	3.83E-02	4.12E-02

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Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by CodySing and last updated on 2024-05-16 11:32:41.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.