D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T296W

Yield (mg/mL): 2.62

**slope of T296W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.20E-03	2.20E-03	2.20E-03
B	25.00	2.20E-03	2.20E-03	2.20E-03
C	8.33
D	2.78	1.95E-03	2.35E-03	2.00E-03
E	0.93	1.05E-03	1.50E-03	1.25E-03
F	0.31	4.50E-04	7.50E-04
G	0.10	3.50E-04	3.00E-04	3.50E-04
H	0.03	2.00E-04	2.00E-04	2.00E-04

Yield (mg/mL): 2.62 Dilution Factor: 100×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-27 21:26:07.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.92E-01	2.95E-01	2.99E-01
B	25.00	2.45E-01	2.34E-01	2.36E-01
C	8.33	1.97E-01	2.14E-01	1.87E-01
D	2.78	1.38E-01	1.42E-01	1.59E-01
E	0.93	5.41E-02	6.23E-02	5.79E-02
F	0.31	2.31E-02	2.16E-02	2.04E-02
G	0.10	7.35E-03	7.85E-03	7.75E-03
H	0.03	2.80E-03	2.55E-03	2.75E-03

Yield (mg/mL): 1.626 Dilution Factor: 100×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 15:28:43.

Rosetta Score for T296W: 2.969

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.