Raw Data for BglB Variant R372F

Expression Data

SDS-PAGE
 0.412

Kinetic Assay Data

slope of R372F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.15E-03 1.10E-03 1.45E-03
B 25.00 1.00E-03 9.50E-04 1.00E-03
C 8.33 8.00E-04 9.00E-04 8.00E-04
D 2.78
E 0.93
F 0.31
G 0.10
H 0.03
 0.412      10×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 16:51:09.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.92E-01 2.95E-01 2.99E-01
B 25.00 2.45E-01 2.34E-01 2.36E-01
C 8.33 1.97E-01 2.14E-01 1.87E-01
D 2.78 1.38E-01 1.42E-01 1.59E-01
E 0.93 5.41E-02 6.23E-02 5.79E-02
F 0.31 2.31E-02 2.16E-02 2.04E-02
G 0.10 7.35E-03 7.85E-03 7.75E-03
H 0.03 2.80E-03 2.55E-03 2.75E-03
 1.626      100×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 15:28:43.


Design Data

5.817

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.