D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R372W

Yield (mg/mL): 0.514

**slope of R372W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.50E-04	3.50E-04	4.00E-04
B	25.00		8.00E-04	5.00E-04
C	8.33	4.00E-04	4.00E-04	3.50E-04
D	2.78	3.00E-04	3.50E-04	3.50E-04
E	0.93	3.00E-04	2.50E-04	4.00E-04
F	0.31	2.00E-04	2.00E-04	1.50E-04
G	0.10	1.00E-04	0.00E+00	1.50E-04
H	0.03	0.00E+00		0.00E+00

Yield (mg/mL): 0.514 Dilution Factor: 10×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 17:20:48.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.96E-01	2.85E-01	2.72E-01
B	25.00	2.40E-01	2.47E-01	2.28E-01
C	8.33	1.86E-01	1.74E-01	1.81E-01
D	2.78	1.37E-01	1.42E-01	1.58E-01
E	0.93	7.40E-02	8.14E-02	7.14E-02
F	0.31	2.50E-02	2.51E-02	2.69E-02
G	0.10	9.60E-03	1.05E-02	8.85E-03
H	0.03	3.75E-03	3.55E-03	3.25E-03

Yield (mg/mL): 1.626 Dilution Factor: 100×

Protein purified on 4/30/2024 and assayed on 5/4/2024.
Data uploaded by RyanAshby123 and last updated on 2024-05-16 17:07:29.

Rosetta Score for R372W: 9.036

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.