Raw Data for BglB Variant L108S

Expression Data

SDS-PAGE
 0.26

Kinetic Assay Data

slope of L108S A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 7.63E+01 7.76E+01
B 25.00 5.97E+01 5.96E+01
C 8.33 5.65E+01 5.61E+01
D 2.78 3.57E+01 2.85E+01
E 0.93 2.41E+01 2.34E+01
F 0.31 1.12E+01 1.03E+01
G 0.10 4.80E-01 1.01E+01
H 0.03 2.70E+00 2.54E+00
 0.26      10×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by brdfong and last updated on 2024-05-22 11:28:01.


Reference Data for WT

slope of WT A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 422.86 461.595 435.472
B 25.00 372.049 374.071 345.585
C 8.33 344.404 334.833 346.619
D 2.78 222.817 196.032 261.951
E 0.93 159.434 156.977
F 0.31 54.931 74.107 102.037
G 0.10 23.077 26.324
H 0.03 9.445 10.588
 1.21      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by lolareimbold and last updated on 2024-05-22 11:17:06.


Thermostability Assay Data

slope of L108S A420 nm (min−1)
T (°C) 1 2 3
A 30.0 3.16E-02 3.74E-02 3.06E-02
B 31.3 3.71E-02 3.38E-02 4.14E-02
C 33.6 3.87E-02 3.39E-02 2.84E-02
D 37.7 2.33E-02 1.53E-02 3.02E-02
E 42.4 1.30E-03 3.20E-03
F 45.7 1.60E-03 1.30E-03 1.00E-03
G 48.3 1.30E-03 9.00E-04 1.00E-03
H 50.0 9.00E-04 1.00E-03

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by brdfong and last updated on 2024-06-05 10:20:36.


Reference Data for WT

slope of L108S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.001200 0.001600 0.001000
B 48.3 0.002100 0.001600 0.002000
C 45.7 0.003100 0.003800 0.005200
D 42.4 0.1133 0.09210 0.1034
E 37.7 0.2491 0.2723
F 33.6 0.3178 0.3007 0.2445
G 31.3 0.2765 0.2451 0.1971
H 30.0 0.2345 0.2210 0.2038

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by lolareimbold and last updated on 2024-05-22 11:26:09.


Design Data

-1.12

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.