Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.21

Kinetic Assay Data

slope of WT A_{420 nm} (10⁻³ min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	422.86	461.595	435.472
	B	25.00	372.049	374.071	345.585
	C	8.33	344.404	334.833	346.619
	D	2.78	222.817	196.032	261.951
	E	0.93		159.434	156.977
	F	0.31	54.931	74.107	102.037
	G	0.10	23.077	26.324
	H	0.03	9.445	10.588

Yield (mg/mL): 1.21     Dilution Factor:  100×

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Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by lolareimbold and last updated on 2024-05-22 11:17:06.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.001200	0.001600	0.001000
	B	48.3	0.002100	0.001600	0.002000
	C	45.7	0.003100	0.003800	0.005200
	D	42.4	0.1133	0.09210	0.1034
	E	37.7	0.2491	0.2723
	F	33.6	0.3178	0.3007	0.2445
	G	31.3	0.2765	0.2451	0.1971
	H	30.0	0.2345	0.2210	0.2038

Download these data

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by lolareimbold and last updated on 2024-05-22 11:26:09.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.