Raw Data for BglB Variant T218S

Expression Data

 1.11

Kinetic Assay Data

slope of T218S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.03E-01 1.15E-01
B 25.00 7.10E-02 6.70E-02
C 8.33 5.20E-02 5.20E-02
D 2.78 2.00E-02 2.00E-02
E 0.93 1.20E-02 1.00E-02
F 0.31 6.00E-03 5.00E-03
G 0.10 2.00E-03 2.00E-03
H 0.03 7.00E-04 7.00E-04
 1.11      100×

Protein purified on 4/15/21 and assayed on 4/22/21.
Data uploaded by Megabr01 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.102 0.092
B 25.00 0.0822 0.0765
C 8.33 0.0718 0.0765
D 2.78 0.0406 0.0412
E 0.93 0.0163 0.0197
F 0.31 0.0086 0.0076
G 0.10 0.0028 0.0034
H 0.03 0.001 0.0009
 0.83      100×

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 16:16:53.


Thermostability Assay Data

slope of T218S A420 nm (min−1)
T (°C) 1 2
A 30.0 4.10E-01 3.41E-01
B 31.8 3.40E-01 2.82E-01
C 33.6 2.97E-01 2.78E-01
D 35.5 0.308 0.297
E 37.3 0.33 0.306
F 39.1 0.279 0.342
G 40.9 0.179 0.176
H 42.7 0.041 0.051
I 44.5 0.019 0.022
J 46.4 0.014 0.013
K 48.2 0.008 0.006
L 50.0 0.006 0.005

Protein purified on 4/15/21 and assayed on 4/22/21.
Data uploaded by Megabr01 and last updated on 2022-07-28 14:17:06.


Reference Data for WT

slope of T218S A420 nm (min−1)
T (°C) 1 2
A 30.0 0.38
B 31.8 0.4374
C 33.6 0.3474
D 35.5 0.3655
E 37.3 0.3835
F 39.1 0.3743
G 40.9 0.22
H 42.7 0.0204
I 44.5 0.0008
J 46.4 0.0003
K 48.1 0
L 50.0 0.0003

Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-07-28 11:05:05.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.