Raw Data for BglB Variant N160V

Expression Data

SDS-PAGE
 1.52

Kinetic Assay Data

slope of N160V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3309 0.3413 0.3542
B 25.00 0.2337 0.2498 0.2540
C 8.33 0.1853 0.1899 0.2022
D 2.78 0.1668 0.1577
E 0.93
F 0.31 0.06265 0.06685 0.08320
G 0.10 0.01220 0.01165 0.01385
H 0.03 0 0 0
 1.52      100×

Protein purified on 24-04-2024 and assayed on 01-05-2024.
Data uploaded by sancharihazra and last updated on 2024-05-22 11:28:14.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.05E-01 3.13E-01 3.09E-01
B 25.00 3.05E-01 2.78E-01 2.81E-01
C 8.33 1.45E-01 1.38E-01
D 2.78 7.85E-02 7.37E-02
E 0.93 2.20E-02 1.55E-02
F 0.31 2.49E-02 2.12E-02
G 0.10 4.90E-03 4.00E-03
H 0.03 9.50E-04 8.00E-04
 1.04      100×

Protein purified on 4/24/24 and assayed on 5/1/24.
Data uploaded by nschen and last updated on 2024-05-22 11:34:00.


Thermostability Assay Data

slope of N160V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0203 0.0023 0.0661
B 48.3 0.0042 0.0025 0.0016
C 45.7 0.0014 0.0014
D 42.4 0.0395 0.0418 0.0402
E 37.7 0.2961 0.2785
F 33.6 0.409 0.3812 0.3507
G 31.3 0.3468 0.3297 0.3301
H 30.0 0.3853 0.3847 0.393

Protein purified on 24-04-2024 and assayed on 08-05-2024.
Data uploaded by sancharihazra and last updated on 2024-05-22 11:37:15.


Reference Data for WT

slope of N160V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0044 0 1.50E-03
B 48.3 2.20E-03 1.20E-03 3.00E-03
C 45.7 1.10E-03 1.80E-03 1.60E-03
D 42.4 7.14E-02 8.61E-02 8.66E-02
E 37.7 2.46E-01 2.46E-01 2.50E-01
F 33.6 2.58E-01 2.47E-01 2.43E-01
G 31.3 2.83E-01 2.92E-01 2.52E-01
H 30.0 2.69E-01 2.82E-01 2.66E-01

Protein purified on 4/24/24 and assayed on 5/8/24.
Data uploaded by nschen and last updated on 2024-05-22 11:37:14.


Design Data

4.151

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.