Raw Data for BglB Variant L171H

Expression Data

SDS-PAGE
 1.02

Kinetic Assay Data

slope of L171H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.64E-01 2.12E-01 2.51E-01
B 25.00 2.10E-01 2.37E-01 2.15E-01
C 8.33 1.47E-01 1.62E-01 1.72E-01
D 2.78 1.00E-01 1.30E-01 1.04E-01
E 0.93 6.12E-02 5.07E-02 4.87E-02
F 0.31 0 0
G 0.10 0 0
H 0.03 0 0
 1.02      100×

Protein purified on 2024/4/24 and assayed on 2024/3/1.
Data uploaded by milong and last updated on 2024-05-22 11:17:26.



Thermostability Assay Data

slope of L171H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 6.00E-04 9.00E-04
B 48.3 8.00E-04 8.00E-04
C 45.7 7.00E-04 7.00E-04
D 42.4 1.04E-02 9.50E-03 8.70E-03
E 37.7 1.18E-01 1.33E-01 1.31E-01
F 33.6 1.29E-01 1.43E-01 1.45E-01
G 31.3 1.66E-01 1.58E-01 1.59E-01
H 30.0 1.72E-01 1.74E-01 1.95E-01

Protein purified on 2024/4/24 and assayed on 2024/5/8.
Data uploaded by milong and last updated on 2024-05-22 11:31:54.


Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.



Design Data

2.945


Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.