Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.04

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.05E-01	3.13E-01	3.09E-01
	B	25.00	3.05E-01	2.78E-01	2.81E-01
	C	8.33		1.45E-01	1.38E-01
	D	2.78		7.85E-02	7.37E-02
	E	0.93		2.20E-02	1.55E-02
	F	0.31		2.49E-02	2.12E-02
	G	0.10		4.90E-03	4.00E-03
	H	0.03		9.50E-04	8.00E-04

Yield (mg/mL): 1.04     Dilution Factor:  100×

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Protein purified on 4/24/24 and assayed on 5/1/24.
Data uploaded by nschen and last updated on 2024-05-22 11:34:00.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0044	0	1.50E-03
	B	48.3	2.20E-03	1.20E-03	3.00E-03
	C	45.7	1.10E-03	1.80E-03	1.60E-03
	D	42.4	7.14E-02	8.61E-02	8.66E-02
	E	37.7	2.46E-01	2.46E-01	2.50E-01
	F	33.6	2.58E-01	2.47E-01	2.43E-01
	G	31.3	2.83E-01	2.92E-01	2.52E-01
	H	30.0	2.69E-01	2.82E-01	2.66E-01

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Protein purified on 4/24/24 and assayed on 5/8/24.
Data uploaded by nschen and last updated on 2024-05-22 11:37:14.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.