Raw Data for BglB Variant T352S

Expression Data

 0.23

Kinetic Assay Data

slope of T352S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0139 0.0148
B 25.00 0.0131 0.0123 0.0152
C 8.33 0.011 0.0093 0.012
D 2.78 0.0054 9.00E-05 0.0077
E 0.93 0.0031 0.0001 0.0046
F 0.31 0.0015 0.0001 0.0029
G 0.10 0.0022 0.0001 0.0011
H 0.03 8.00E-05 5.00E-05
 0.23      100×

Protein purified on 4/15/21 and assayed on 4/22/21.
Data uploaded by stephaniematlaga and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.128 0.137
B 25.00 0.130 0.129
C 8.33 0.110 0.100
D 2.78 0.080 0.086
E 0.93 0.065 0.047
F 0.31 0.040 0.031
G 0.10 0.025 0.016
H 0.03 0
 1.38      100×

Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of T352S A420 nm (min−1)
T (°C) 1 2
A 30.0 0.0403 0.039
B 31.8 4.34E-02 4.68E-02
C 33.6 3.10E-02 4.48E-02
D 35.5 4.55E-02 0.0433
E 37.3 2.63E-02 0.0085
F 39.1 1.44E-02 0.0135
G 40.9 0.001 0.001
H 42.7 0 0.0001
I 44.5 0 0
J 46.4 0 0
K 48.1 0 0
L 50.0 0 0

Protein purified on 4/15/21 and assayed on 4/22/21.
Data uploaded by stephaniematlaga and last updated on 2022-07-28 14:21:04.


Reference Data for WT

slope of T352S A420 nm (min−1)
T (°C) 1 2
A 30.0 0.38
B 31.8 0.4374
C 33.6 0.3474
D 35.5 0.3655
E 37.3 0.3835
F 39.1 0.3743
G 40.9 0.22
H 42.7 0.0204
I 44.5 0.0008
J 46.4 0.0003
K 48.1 0
L 50.0 0.0003

Protein purified on 4/13/2021 and assayed on 4/22/2021.
Data uploaded by Canoma01 and last updated on 2022-07-28 11:05:05.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.