D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R297A

Expression Data

Yield (mg/mL): 0.62

Kinetic Assay Data

**slope of R297A A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.81E+01	1.20E+02
B	25.00	7.92E+01	8.12E+01	8.33E+01
C	8.33	6.19E+01	7.05E+01
D	2.78	6.96E+01	4.93E+01	5.77E+01
E	0.93	2.54E+01		2.34E+01
F	0.31	1.02E+01	9.70E+00	9.63E+00
G	0.10	3.62E+00	4.12E+00	4.27E+00
H	0.03	2.01E+00		2.94E+00

Yield (mg/mL): 0.62 Dilution Factor: 10×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by lmathios and last updated on 2024-05-22 14:58:27.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.76E+02	2.68E+02	2.95E+02
B	25.00	2.44E+02	2.43E+02	2.46E+02
C	8.33	2.36E+02	2.62E+02	2.54E+02
D	2.78	1.80E+02	1.60E+02	1.79E+02
E	0.93	6.16E+01	6.37E+01	5.80E+01
F	0.31	4.75E-01	2.83E+01	3.83E+01
G	0.10	1.25E+01	1.39E+01	1.20E+01
H	0.03	5.36E+00	5.34E+00	5.51E+00

Yield (mg/mL): 0.89 Dilution Factor: 100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by dlivira and last updated on 2024-05-22 14:34:47.

Thermostability Assay Data

**slope of R297A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	4.14E-01	0	6.35E-01
B	48.3		0	0
C	45.7	1.57E+00	1.59E+00	1.85E+00
D	42.4	4.61E+01	4.14E+01	4.22E+01
E	37.7	1.07E+02	1.05E+02	1.06E+02
F	33.6	1.04E+02	1.14E+02	1.12E+02
G	31.3	1.12E+02	1.07E+02	1.09E+02
H	30.0	1.12E+02	1.08E+02	1.01E+02

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by lmathios and last updated on 2024-05-22 14:57:51.

Reference Data for WT

**slope of R297A A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		3.96E-01	3.48E-01
B	48.3		3.72E-01	7.52E-01
C	45.7	1.93E+00	1.73E+00	2.46E+00
D	42.4	1.10E+02	1.09E+02	1.36E+02
E	37.7	3.65E+02	3.37E+02	3.56E+02
F	33.6	4.10E+02	3.62E+02	2.54E+02
G	31.3	3.63E+02	3.55E+02	3.55E+02
H	30.0	3.53E+02	3.64E+02	3.66E+02

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by dlivira and last updated on 2024-05-22 14:42:57.

Design Data

Rosetta Score for R297A: 5.894

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.