Raw Data for BglB Variant T296R

Expression Data

SDS-PAGE
 0.91

Kinetic Assay Data

slope of T296R A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 2.12E+02 1.86E+02 1.97E+02
B 25.00
C 8.33 1.79E+02 2.10E+02 1.86E+02
D 2.78 1.54E+02 1.71E+02 1.32E+02
E 0.93 8.19E+01 8.20E+01 7.69E+01
F 0.31 4.32E+01 3.78E+01 3.27E+01
G 0.10 1.84E+01 1.64E+01 1.79E+01
H 0.03 1.34E+00
 0.91      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by njyau and last updated on 2024-05-22 14:33:21.


Reference Data for WT

slope of WT A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 2.59E+02 2.71E+02 2.64E+02
B 25.00
C 8.33 1.83E+02 2.03E+02
D 2.78 168.737 1.67E+02 2.05E+02
E 0.93 9.42E+01 1.01E+02 8.51E+01
F 0.31 4.03E+01 4.79E+01 4.77E+01
G 0.10 1.45E+01 1.72E+01 1.93E+01
H 0.03 3.66E-01 1.97E+00
 0.65      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by wesyu and last updated on 2024-05-22 14:32:58.


Thermostability Assay Data

slope of T296R A420 nm (10−3 min−1)
T (°C) 1 2 3
A 30.0 2.41E+02 2.64E+02 2.58E+02
B 31.3 2.94E+02 2.98E+02 2.98E+02
C 33.6 2.57E+02 2.80E+02 3.00E+02
D 37.7 2.94E+02 3.02E+02 3.13E+02
E 42.4 1.59E+02 1.66E+02 1.37E+02
F 45.7 5.83E+00 5.05E+00 4.43E+00
G 48.3 1.17E+00 1.17E+00 1.18E+00
H 50.0 9.87E-01 7.78E-01

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by njyau and last updated on 2024-05-22 14:19:08.


Reference Data for WT

slope of T296R A420 nm (10−3 min−1)
T (°C) 1 2 3
A 30.0 3.26E+02 2.95E+02 3.34E+02
B 31.3 3.76E+02 3.35E+02 3.19E+02
C 33.6 3.01E+02 2.76E+02
D 37.7 3.25E+02 3.95E+02 3.16E+02
E 42.4 9.18E+01 8.73E+01 9.28E+01
F 45.7 2.23E+00 2.37E+00
G 48.3 8.30E-01 9.94E-01 5.86E-01
H 50.0 6.05E-01 3.94E-01

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by wesyu and last updated on 2024-05-22 14:17:56.


Design Data

0.769

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.