Raw Data for BglB Variant S298T

Expression Data

SDS-PAGE
 0.847

Kinetic Assay Data

slope of S298T A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.91E-01 1.86E-01 1.80E-01
B 25.00 1.58E-01 1.64E-01 1.57E-01
C 8.33 1.47E-01 1.62E-01 1.55E-01
D 2.78 6.34E-02 7.52E-02 9.21E-02
E 0.93 3.50E-02 4.21E-02 4.38E-02
F 0.31 1.75E-02 2.36E-02 1.85E-02
G 0.10 6.00E-03 8.70E-03 8.00E-03
H 0.03 2.10E-03 2.80E-03 2.80E-03
 0.847      100×

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.102 0.092
B 25.00 0.0822 0.0765
C 8.33 0.0718 0.0765
D 2.78 0.0406 0.0412
E 0.93 0.0163 0.0197
F 0.31 0.0086 0.0076
G 0.10 0.0028 0.0034
H 0.03 0.001 0.0009
 0.83      100×

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 16:16:53.


Thermostability Assay Data

slope of S298T A420 nm (min−1)
T (°C) 1 2
A 30.0 4.65E-01
B 31.8 5.69E-01 5.49E-01
C 33.6 6.21E-01 7.62E-01
D 35.5 0.5166 0.7604
E 37.3 0.9363 0.6856
F 39.1 0.4466 0.5255
G 40.9 0.0479 0.0421
H 42.7 0.0014 0.001
I 44.5 0.0002 0.00007
J 46.4 0.0001 0
K 48.1 0 0
L 50.0 0 0

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 14:28:38.


Reference Data for WT

slope of S298T A420 nm (min−1)
T (°C) 1 2
A 30.0 3.80E-01
B 31.8 4.37E-01
C 33.6 3.47E-01
D 35.5 0.3655
E 37.3 0.3835
F 39.1 0.3743
G 40.9 0.22
H 42.7 0.0204
I 44.5 0.0008
J 46.4 0.0003
K 48.1 0
L 50.0 0

Protein purified on 4/15/2021 and assayed on 4/22/2021.
Data uploaded by grahno02 and last updated on 2022-07-28 16:17:05.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.