Raw Data for BglB Variant W120E

Expression Data

SDS-PAGE
 0.11

Kinetic Assay Data

slope of W120E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.0032 0.0033 0.0033
B 25.00 0.0023 0.0022 0.0024
C 8.33 0.0023 0.0025 0.0021
D 2.78 0.0015 0.0014
E 0.93 0.0013
F 0.31 0.0013
G 0.10
H 0.03 0 0
 0.11      10×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by Mcaldm and last updated on 2024-05-22 14:28:28.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.92E-01 3.30E-01 3.42E-01
B 25.00 2.30E-01 2.88E-01 2.80E-01
C 8.33 2.08E-01 2.44E-01 2.41E-01
D 2.78 1.84E-01 1.47E-01 1.59E-01
E 0.93 7.64E-02 7.66E-02
F 0.31 2.92E-02 3.95E-02 2.84E-02
G 0.10 1.38E-02 1.02E-02 1.04E-02
H 0.03 5.80E-03 4.90E-03 5.55E-03
 1.52      100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by jmjha and last updated on 2024-05-22 14:39:16.


Thermostability Assay Data

slope of W120E A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.28100 0.2783000 0.265800
B 31.3 0.2896000 0.271200 0.2858000
C 33.6 0.270700 0.271800 0.270400
D 37.7 0.25780 0.24460 0.25090
E 42.4 0.0847 0.0844 0.0793
F 45.7 0.0027 0.0022 0.0021
G 48.3 0.0011 0.0011
H 50.0 0 0

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by Mcaldm and last updated on 2024-05-22 14:40:11.


Reference Data for WT

slope of W120E A420 nm (min−1)
T (°C) 1 2 3
A 30.0 3.97E-01 3.67E-01 3.72E-01
B 31.3 3.82E-01 3.73E-01 3.63E-01
C 33.6 3.83E-01 3.66E-01 3.39E-01
D 37.7 3.54E-01 3.33E-01 3.34E-01
E 42.4 1.01E-01 9.26E-02 1.01E-01
F 45.7 1.20E-03 1.60E-03
G 48.3 4.50E-03 2.30E-03
H 50.0

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by jmjha and last updated on 2024-05-22 14:34:26.


Design Data

6.469

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.