Raw Data for BglB Variant H315Q

Expression Data

SDS-PAGE
 0.41

Kinetic Assay Data

slope of H315Q A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 6.24E-01 1.25E+00
C 8.33 5.86E-01 5.86E-01
D 2.78 5.98E-01 2.26E-01
E 0.93 3.89E-01 3.98E-01
F 0.31 1.93E-01 3.80E-01
G 0.10 1.98E-01 2.01E-01
H 0.03 1.86E-01
 0.41      100×

Protein purified on 4/24/24 and assayed on 5/1/24.
Data uploaded by Stephanie and last updated on 2024-12-19 13:55:19.


Reference Data for WT

slope of WT A420 nm (10−3 min−1)
[S] (mm) 1 2 3
A 75.00 2.59E+02 2.71E+02 2.64E+02
B 25.00
C 8.33 1.83E+02 2.03E+02
D 2.78 168.737 1.67E+02 2.05E+02
E 0.93 9.42E+01 1.01E+02 8.51E+01
F 0.31 4.03E+01 4.79E+01 4.77E+01
G 0.10 1.45E+01 1.72E+01 1.93E+01
H 0.03 3.66E-01 1.97E+00
 0.65      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by wesyu and last updated on 2024-05-22 14:32:58.


Thermostability Assay Data

slope of H315Q A420 nm (min−1)
T (°C) 1 2 3
A 30.0 1.36E+01 1.59E+01 1.62E+01
B 31.3 1.45E+01 1.45E+01 1.80E+01
C 33.6 1.52E+01 1.65E+01 1.67E+01
D 37.7 1.27E+01 1.52E+01 1.96E+01
E 42.4 4.49E+00 3.58E+00 3.56E+00
F 45.7 8.07E-01 7.83E-01
G 48.3 7.65E-01
H 50.0 7.67E-01

Protein purified on 4/24/24 and assayed on 5/8/24.
Data uploaded by Stephanie and last updated on 2024-05-23 21:03:34.


Reference Data for WT

slope of H315Q A420 nm (10−3 min−1)
T (°C) 1 2 3
A 30.0 3.26E+02 2.95E+02 3.34E+02
B 31.3 3.76E+02 3.35E+02 3.19E+02
C 33.6 3.01E+02 2.76E+02
D 37.7 3.25E+02 3.95E+02 3.16E+02
E 42.4 9.18E+01 8.73E+01 9.28E+01
F 45.7 2.23E+00 2.37E+00
G 48.3 8.30E-01 9.94E-01 5.86E-01
H 50.0 6.05E-01 3.94E-01

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by wesyu and last updated on 2024-05-22 14:17:56.


Design Data

-1.133

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.