Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.92E-01	3.30E-01	3.42E-01
	B	25.00	2.30E-01	2.88E-01	2.80E-01
	C	8.33	2.08E-01	2.44E-01	2.41E-01
	D	2.78	1.84E-01	1.47E-01	1.59E-01
	E	0.93	7.64E-02		7.66E-02
	F	0.31	2.92E-02	3.95E-02	2.84E-02
	G	0.10	1.38E-02	1.02E-02	1.04E-02
	H	0.03	5.80E-03	4.90E-03	5.55E-03

Yield (mg/mL): 1.52     Dilution Factor:  100×

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Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by jmjha and last updated on 2024-05-22 14:39:16.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	3.97E-01	3.67E-01	3.72E-01
	B	31.3	3.82E-01	3.73E-01	3.63E-01
	C	33.6	3.83E-01	3.66E-01	3.39E-01
	D	37.7	3.54E-01	3.33E-01	3.34E-01
	E	42.4	1.01E-01	9.26E-02	1.01E-01
	F	45.7		1.20E-03	1.60E-03
	G	48.3	4.50E-03	2.30E-03
	H	50.0

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Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by jmjha and last updated on 2024-05-22 14:34:26.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.