D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y173L

Expression Data

Yield (mg/mL): 0.17

Kinetic Assay Data

**slope of Y173L A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		1.04E+01	9.54E+00
C	8.33	8.42E+00	8.07E+00	6.70E+00
D	2.78	7.05E+00	6.98E+00	7.02E+00
E	0.93	4.07E+00	3.82E+00	3.99E+00
F	0.31	2.15E+00	1.72E+00	2.14E+00
G	0.10		7.72E-01	9.75E-01
H	0.03

Yield (mg/mL): 0.17 Dilution Factor: 100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by Catvenom and last updated on 2024-05-22 14:23:57.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.59E+02	2.71E+02	2.64E+02
B	25.00
C	8.33		1.83E+02	2.03E+02
D	2.78	168.737	1.67E+02	2.05E+02
E	0.93	9.42E+01	1.01E+02	8.51E+01
F	0.31	4.03E+01	4.79E+01	4.77E+01
G	0.10	1.45E+01	1.72E+01	1.93E+01
H	0.03	3.66E-01		1.97E+00

Yield (mg/mL): 0.65 Dilution Factor: 100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by wesyu and last updated on 2024-05-22 14:32:58.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of Y173L A_{420 nm} (10⁻³ min⁻¹)**
	T (°C)	1	2	3
A	30.0	3.26E+02	2.95E+02	3.34E+02
B	31.3	3.76E+02	3.35E+02	3.19E+02
C	33.6		3.01E+02	2.76E+02
D	37.7	3.25E+02	3.95E+02	3.16E+02
E	42.4	9.18E+01	8.73E+01	9.28E+01
F	45.7	2.23E+00	2.37E+00
G	48.3	8.30E-01	9.94E-01	5.86E-01
H	50.0	6.05E-01	3.94E-01

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by wesyu and last updated on 2024-05-22 14:17:56.

Design Data

Rosetta Score for Y173L: -3.625

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.