Raw Data for BglB Variant Y75N

Expression Data

SDS-PAGE
 0.09

Kinetic Assay Data

slope of Y75N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 0.0078 0.0084 0.0075
C 8.33 0.0070 0.0072 0.0061
D 2.78 0.0046 0.0049 0.0049
E 0.93 0.00265 0.00215
F 0.31 0.00130 0.00125 0.00120
G 0.10 0.000950 0.001100 0.001150
H 0.03 0.001050 0.000900 0.001300
 0.09      100×

Protein purified on 4/25/2024 and assayed on 5/1/2024.
Data uploaded by AnnikaS and last updated on 2024-05-22 14:05:43.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4104 0.3731 0.4015
B 25.00 0.2936 0.3667 0.3656
C 8.33 0.3006 0.2905 0.2660
D 2.78 0.1869 0.1915 0.1908
E 0.93 0.08815 0.09025 0.08730
F 0.31 0.03495 0.03495 0.03200
G 0.10 0.013100 0.014900 0.013900
H 0.03 0.006750 0.006300 0.008400
 1.37      100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:56:57.


Thermostability Assay Data

slope of Y75N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00070 0.0017000 0.001100
B 48.3
C 45.7 0.001000 0.000900
D 42.4 0.00100
E 37.7 0.0018 0.0015 0.0023
F 33.6 0.0020 0.0021 0.0023
G 31.3 0.0032 0.0029 0.0036
H 30.0 0.0041 0.0045 0.0040

Protein purified on 4/25/2024 and assayed on 5/8/2024.
Data uploaded by AnnikaS and last updated on 2024-05-22 14:09:25.


Reference Data for WT

slope of Y75N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00150 0 0.000500
B 48.3 0.0025000 0.001800 0.0020000
C 45.7 0.010800 0.010200
D 42.4 0.12360 0.11550
E 37.7 0.4620 0.4222 0.4359
F 33.6 0.5037 0.4569 0.4778
G 31.3 0.4306 0.4816 0.4612
H 30.0 0.4487 0.4498 0.4508

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:58:20.


Design Data

10.766

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.