D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N354S

Expression Data

Yield (mg/mL): 0.25

Kinetic Assay Data

**slope of N354S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0052	0.0050	0.0049
B	25.00	0.0042	0.0043	0.0056
C	8.33	0.0034	0.0038	0.0035
D	2.78	0.0029	0.0029	0.0028
E	0.93	0.00150	0.00140	0.00150
F	0.31	0.00110		0.00090
G	0.10	0.000950	0.000750	0.001000
H	0.03

Yield (mg/mL): 0.25 Dilution Factor: 100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by gwynethlb and last updated on 2024-05-22 14:07:57.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4104	0.3731	0.4015
B	25.00	0.2936	0.3667	0.3656
C	8.33	0.3006	0.2905	0.2660
D	2.78	0.1869	0.1915	0.1908
E	0.93	0.08815	0.09025	0.08730
F	0.31	0.03495	0.03495	0.03200
G	0.10	0.013100	0.014900	0.013900
H	0.03	0.006750	0.006300	0.008400

Yield (mg/mL): 1.37 Dilution Factor: 100×

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:56:57.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of N354S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.00150	0	0.000500
B	48.3	0.0025000	0.001800	0.0020000
C	45.7		0.010800	0.010200
D	42.4	0.12360	0.11550
E	37.7	0.4620	0.4222	0.4359
F	33.6	0.5037	0.4569	0.4778
G	31.3	0.4306	0.4816	0.4612
H	30.0	0.4487	0.4498	0.4508

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:58:20.

Design Data

Rosetta Score for N354S: 1.827

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.