Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.37

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.4104	0.3731	0.4015
	B	25.00	0.2936	0.3667	0.3656
	C	8.33	0.3006	0.2905	0.2660
	D	2.78	0.1869	0.1915	0.1908
	E	0.93	0.08815	0.09025	0.08730
	F	0.31	0.03495	0.03495	0.03200
	G	0.10	0.013100	0.014900	0.013900
	H	0.03	0.006750	0.006300	0.008400

Yield (mg/mL): 1.37     Dilution Factor:  100×

Download these data

Protein purified on 4/24/2024 and assayed on 5/1/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:56:57.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00150	0	0.000500
	B	48.3	0.0025000	0.001800	0.0020000
	C	45.7		0.010800	0.010200
	D	42.4	0.12360	0.11550
	E	37.7	0.4620	0.4222	0.4359
	F	33.6	0.5037	0.4569	0.4778
	G	31.3	0.4306	0.4816	0.4612
	H	30.0	0.4487	0.4498	0.4508

Download these data

Protein purified on 4/24/2024 and assayed on 5/8/2024.
Data uploaded by ebennet and last updated on 2024-05-22 14:58:20.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.