D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y333F

Expression Data

Yield (mg/mL): 2.736

Kinetic Assay Data

**slope of Y333F A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	252.1000	314.3000	294.5000
B	25.00		217.7000	219.2000
C	8.33		173.9000	175.9000
D	2.78		141.9000	163.9000
E	0.93		105.40000	103.20000
F	0.31		62.50000	56.30000
G	0.10		39.500000	42.900000
H	0.03		16.300000	17.700000

Yield (mg/mL): 2.736 Dilution Factor: 100×

Protein purified on 5/13/2024 and assayed on 5/15/2024.
Data uploaded by lfarinsky and last updated on 2024-06-27 10:24:25.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	220.9000	259.9000	254.0000
B	25.00	202.7000	207.8000	200.4000
C	8.33	145.6000	137.3000	124.9000
D	2.78	34.8000	34.1000	39.2000
E	0.93	16.40000	8.80000	14.10000
F	0.31	5.00000	4.00000	5.40000
G	0.10	2.100000	2.400000	3.000000
H	0.03	0.900000	0.800000	0.900000

Yield (mg/mL): 2.016 Dilution Factor: 100×

Protein purified on 5/13/2024 and assayed on 5/15/2024.
Data uploaded by lfarinsky and last updated on 2024-06-27 10:41:06.

Design Data

Rosetta Score for Y333F: 0.322

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.