Raw Data for BglB Variant L171Y

Expression Data

 0.914

Kinetic Assay Data

slope of L171Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.102 0.110 0.104
B 25.00 0.106 0.111 0.116
C 8.33 0.115 0.102 0.0857
D 2.78 0.0834 0.0734 0.0699
E 0.93 0.0541 0.0510 0.0506
F 0.31 0.0284 0.0249 0.0217
G 0.10 0.0126 0.0111 0.00970
H 0.03 0.00550 0.00500 0.00420
 0.914      100×

Protein purified on 4/16/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.114 0.120
B 25.00 0.0790 0.0784
C 8.33 0.0304 0.0836
D 2.78 0.0467 0.0489
E 0.93 0.0407 0.0330
F 0.31 0.0141 0.0139
G 0.10 0.00520 0.00360
H 0.03 0.00460 0.00330
 0.834      100×

Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of L171Y A420 nm (min−1)
T (°C) 1 2
A 30.0 6.25E-01 6.36E-01
B 31.8 6.40E-01 6.50E-01
C 33.6 5.55E-01 5.64E-01
D 35.5 0.59 0.584
E 37.3 0.306 0.281
F 39.1 0.0704 0.0601
G 40.9 0.0037 0.0036
H 42.7 0.0003 0.0005
I 44.5 0.000000 0.0002
J 46.4 0 0
K 48.1 0 0.0001
L 50.0 0 0

Protein purified on 4/16/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-07-28 13:47:03.


Reference Data for WT

slope of L171Y A420 nm (min−1)
T (°C) 1 2
A 30.0 6.65E-01
B 31.8 7.93E-01
C 33.6 7.79E-01
D 35.5 0.639
E 37.3 0.724
F 39.1 0.677
G 40.9 0.259
H 42.7 0.0309
I 44.5 0.0012
J 46.4 0.0004
K 48.1 0.0003
L 50.0 0.0002

Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-07-28 13:47:07.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.