D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A88E

Expression Data

Yield (mg/mL): 2.138

Kinetic Assay Data

**slope of A88E A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	212.1000	179.1000	199.5000
B	25.00	149.9000	133.9000	129.9000
C	8.33	122.1000	127.2000	128.8000
D	2.78	113.9000	123.0000	134.9000
E	0.93	101.30000	93.90000	106.50000
F	0.31	48.20000	59.20000	63.50000
G	0.10	35.300000	37.300000	34.000000
H	0.03	14.000000	15.700000	13.200000

Yield (mg/mL): 2.138 Dilution Factor: 100×

Protein purified on 5/13/2024 and assayed on 5/15/2024.
Data uploaded by lfarinsky and last updated on 2024-06-27 10:37:26.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	220.9000	259.9000	254.0000
B	25.00	202.7000	207.8000	200.4000
C	8.33	145.6000	137.3000	124.9000
D	2.78	34.8000	34.1000	39.2000
E	0.93	16.40000	8.80000	14.10000
F	0.31	5.00000	4.00000	5.40000
G	0.10	2.100000	2.400000	3.000000
H	0.03	0.900000	0.800000	0.900000

Yield (mg/mL): 2.016 Dilution Factor: 100×

Protein purified on 5/13/2024 and assayed on 5/15/2024.
Data uploaded by lfarinsky and last updated on 2024-06-27 10:41:06.

Design Data

Rosetta Score for A88E: -1.443

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.