Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.558

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00
	B	25.00
	C	8.33
	D	2.78	1.63E-01	2.10E-01
	E	0.93	1.34E-01	8.85E-02
	F	0.31	4.25E-02	2.90E-02
	G	0.10	1.70E-02	1.10E-02
	H	0.03	6.50E-03	4.00E-03

Yield (mg/mL): 5.558     Dilution Factor:  100×

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Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 14:25:41.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.30	0.30
	B	48.3	0.30	0.40
	C	45.7	0.90	0.80	0.80
	D	42.4		53.70	56.00
	E	37.7
	F	33.6
	G	31.3
	H	30.0

Download these data

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:17:54.

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.