Raw Data for BglB Variant E388Y

Expression Data

SDS-PAGE
 3.41

Kinetic Assay Data

slope of E388Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 1.40E-01 1.54E-01 1.53E-01
E 0.93 5.60E-02 6.40E-02 5.80E-02
F 0.31 2.05E-02 2.95E-02 2.80E-02
G 0.10 1.20E-02 9.50E-03 9.00E-03
H 0.03 5.50E-03 1.00E-03 3.00E-03
 3.41      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by tcris and last updated on 2024-06-13 16:16:47.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 1.63E-01 2.10E-01
E 0.93 1.34E-01 8.85E-02
F 0.31 4.25E-02 2.90E-02
G 0.10 1.70E-02 1.10E-02
H 0.03 6.50E-03 4.00E-03
 5.558      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 14:25:41.


Thermostability Assay Data

slope of E388Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 2.00E-01 2.00E-01
B 48.3 3.00E-01 2.00E-01 0
C 45.7 4.00E-01 4.00E-01
D 42.4 6.30E+00 8.00E+00 7.80E+00
E 37.7 2.03E+02 2.25E+02
F 33.6 4.44E+02 4.73E+02
G 31.3 4.55E+02
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by tcris and last updated on 2024-06-13 17:28:58.


Reference Data for WT

slope of E388Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.40
C 45.7 0.90 0.80 0.80
D 42.4 53.70 56.00
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:17:54.


Design Data

35.697

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.