Raw Data for BglB Variant N293V

Expression Data

SDS-PAGE
 5.69

Kinetic Assay Data

slope of N293V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.30E-02 1.20E-02 1.25E-02
B 25.00 1.15E-02 1.10E-02 1.15E-02
C 8.33 7.50E-03 7.50E-03 7.50E-03
D 2.78 3.50E-03 4.00E-03 4.00E-03
E 0.93 0.00E+00 2.00E-03
F 0.31 5.00E-04 5.00E-04
G 0.10 5.00E-04 5.00E-04 5.00E-04
H 0.03 5.00E-04 5.00E-04
 5.69      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by epei679 and last updated on 2024-06-13 15:22:32.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 2.08E-01
E 0.93 1.44E-01 1.25E-01 1.55E-01
F 0.31 7.50E-02 8.25E-02 1.00E-01
G 0.10 3.85E-02 4.80E-02 4.85E-02
H 0.03 2.10E-02 1.65E-02 2.65E-02
 5.636      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 15:22:35.


Thermostability Assay Data

slope of N293V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.40
B 48.3 0.20 0.20
C 45.7 0 0.50 1.00
D 42.4 0.50 0.50
E 37.7 12.30 15.10 13.00
F 33.6 12.20 18.30 16.60
G 31.3 17.80 16.40 17.10
H 30.0 16.00 15.80 17.90

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by epei679 and last updated on 2024-06-13 18:23:51.


Reference Data for WT

slope of N293V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.40
C 45.7 0.90 0.80 0.80
D 42.4 53.70 56.00
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:17:54.


Design Data

1.015

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.