Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 6.66

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00
	B	25.00
	C	8.33
	D	2.78
	E	0.93	1.77E-01	1.69E-01	1.45E-01
	F	0.31	6.05E-02	5.95E-02	5.45E-02
	G	0.10	2.25E-02	2.15E-02	1.75E-02
	H	0.03	7.00E-03	6.50E-03	7.00E-03

Yield (mg/mL): 6.66     Dilution Factor:  100×

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Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 15:20:29.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.30	0.30
	B	48.3		0.30	0.40
	C	45.7	1.30	1.20	0.90
	D	42.4	59.90	73.50	70.60
	E	37.7
	F	33.6
	G	31.3
	H	30.0

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Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:21:19.

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.