Raw Data for BglB Variant N293V

Expression Data

SDS-PAGE
 4.286

Kinetic Assay Data

slope of N293V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.00E-02 1.00E-02 1.00E-02
B 25.00 1.00E-02 1.00E-02
C 8.33 6.50E-03 7.50E-03 7.00E-03
D 2.78 3.50E-03 4.00E-03 3.50E-03
E 0.93 1.50E-03 1.50E-03 1.50E-03
F 0.31 5.00E-04 5.00E-04
G 0.10 5.00E-04 5.00E-04 5.00E-04
H 0.03 0.00E+00 5.00E-04 1.50E-03
 4.286      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by epei679 and last updated on 2024-06-13 15:38:06.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93 1.77E-01 1.69E-01 1.45E-01
F 0.31 6.05E-02 5.95E-02 5.45E-02
G 0.10 2.25E-02 2.15E-02 1.75E-02
H 0.03 7.00E-03 6.50E-03 7.00E-03
 6.66      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 15:20:29.


Thermostability Assay Data

slope of N293V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0.20 0.30
C 45.7 0 0
D 42.4 0.50 0.40 0.40
E 37.7 3.70 4.10
F 33.6 6.00 8.90 7.20
G 31.3 8.70 9.50 10.60
H 30.0 8.70 8.00

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by epei679 and last updated on 2024-06-13 19:05:56.


Reference Data for WT

slope of N293V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.40
C 45.7 1.30 1.20 0.90
D 42.4 59.90 73.50 70.60
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:21:19.


Design Data

1.015

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.