Raw Data for BglB Variant Q139E

Expression Data

SDS-PAGE
 5.149

Kinetic Assay Data

slope of Q139E A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78 2.44E-01 2.24E-01 2.30E-01
E 0.93 1.02E-01 9.55E-02 1.04E-01
F 0.31 3.70E-02 3.65E-02 3.75E-02
G 0.10 1.35E-02 1.10E-02 1.20E-02
H 0.03 4.50E-03 4.00E-03 4.50E-03
 5.149      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by Ben and last updated on 2024-06-13 16:03:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00
C 8.33
D 2.78
E 0.93 1.77E-01 1.69E-01 1.45E-01
F 0.31 6.05E-02 5.95E-02 5.45E-02
G 0.10 2.25E-02 2.15E-02 1.75E-02
H 0.03 7.00E-03 6.50E-03 7.00E-03
 6.66      100×

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 15:20:29.


Thermostability Assay Data

slope of Q139E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.40 0.60 0.50
B 48.3 0.40 0.30 0.60
C 45.7 1.50 1.50 1.30
D 42.4 0.30 132.70 79.30
E 37.7 675.10 627.20
F 33.6 716.60 718.40
G 31.3 711.10 824.20
H 30.0 671.90 808.40

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by Ben and last updated on 2024-06-13 17:37:32.


Reference Data for WT

slope of Q139E A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.30 0.30
B 48.3 0.30 0.40
C 45.7 1.30 1.20 0.90
D 42.4 59.90 73.50 70.60
E 37.7
F 33.6
G 31.3
H 30.0

Protein purified on 5/20/2024 and assayed on 5/22/2024.
Data uploaded by dest951 and last updated on 2024-06-13 17:21:19.


Design Data

-0.563

Citations

The unpublished data above were collected by students from La Sierra University. For inquiries, please contact Jesica Jones: <jjones1@lasierra.edu>.