Raw Data for BglB Variant E127D

Expression Data

SDS-PAGE
 0.219

Kinetic Assay Data

slope of E127D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.34E-02 9.34E-02 7.86E-02
B 25.00 9.23E-02 7.01E-02 6.92E-02
C 8.33 7.83E-02 6.80E-02 6.53E-02
D 2.78 4.29E-02 4.76E-02 4.66E-02
E 0.93 2.39E-02 2.62E-02 2.79E-02
F 0.31 8.10E-03 1.27E-02 9.50E-03
G 0.10 2.40E-03 7.20E-03 5.10E-03
H 0.03 1.80E-03 1.80E-03
 0.219      10×

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by tubeke01 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.114 0.120
B 25.00 0.0790 0.0784
C 8.33 0.0304 0.0836
D 2.78 0.0467 0.0489
E 0.93 0.0407 0.0330
F 0.31 0.0141 0.0139
G 0.10 0.00520 0.00360
H 0.03 0.00460 0.00330
 0.834      100×

Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of E127D A420 nm (min−1)
T (°C) 1 2
A 30.0 2.54E-01 3.57E-01
B 31.8 4.10E-01 4.05E-01
C 33.6 2.94E-01 2.86E-01
D 35.5 0.315 0.3173
E 37.3 0.2937 0.3173
F 39.1 0.0906 0.2594
G 40.9 0.0906 0.0916
H 42.7 0.003 0.0018
I 44.5 0.0002 0.0004
J 46.4 0 0
K 48.1 0.0288 0
L 50.0 0 0

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by tubeke01 and last updated on 2022-07-28 15:04:51.


Reference Data for WT

slope of E127D A420 nm (min−1)
T (°C) 1 2
A 30.0 0.664885714
B 31.8 0.79277
C 33.6 0.77854
D 35.5 0.639442857
E 37.3 0.7241
F 39.1 0.67721
G 40.9 0.258779341
H 42.7 0.03094044
I 44.5 0.00119706
J 46.4 0.00043618
K 48.1 0.00025809
L 50.0 0.00022632

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-07-28 11:26:16.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.