Raw Data for BglB Variant N220D

Expression Data

Kinetic Assay Data

slope of N220D A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.78E-02	3.72E-02	3.78E-02
	B	25.00	3.44E-02	3.48E-02	3.74E-02
	C	8.33	2.36E-02	2.59E-02	2.31E-02
	D	2.78	1.58E-02	1.64E-02	1.64E-02
	E	0.93	5.10E-03	4.65E-03	5.40E-03
	F	0.31	2.70E-03	2.25E-03	2.10E-03
	G	0.10	1.65E-03	1.95E-03	1.65E-03
	H	0.03	0		0

Yield (mg/mL): 1.535     Dilution Factor:  10×

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Protein purified on 3/27/2023 and assayed on 3/27/2023.
Data uploaded by mlope618 and last updated on 2024-06-26 12:29:27.

Thermostability Assay Data

slope of N220D A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	9.00E-04	6.00E-04	7.50E-04
	B	48.3	7.50E-04	6.00E-04	7.50E-04
	C	45.7	4.50E-04		6.00E-04
	D	42.4	7.50E-04	7.50E-04	9.00E-04
	E	37.7	6.75E-03	6.60E-03	6.00E-03
	F	33.6	2.49E-02	2.69E-02	2.46E-02
	G	31.3	3.32E-02	3.14E-02	3.03E-02
	H	30.0	3.20E-02	2.97E-02	3.09E-02

Download these data

Protein purified on 3/27/2023 and assayed on 3/27/2023.
Data uploaded by mlope618 and last updated on 2024-06-26 12:34:08.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Janelle Nunez-Castilla: <jnune033@fiu.edu>.