D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W399Q

Expression Data

Yield (mg/mL): 3.85

Kinetic Assay Data

**slope of W399Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.18E-03	4.76E-03	4.69E-03
B	25.00	3.31E-03	4.28E-03	3.17E-03
C	8.33	1.98E-03	1.37E-03	1.58E-03
D	2.78	6.40E-04	6.60E-04	6.00E-04
E	0.93	2.50E-04	2.40E-04	2.20E-04
F	0.31	1.60E-04	1.30E-04	1.40E-04
G	0.10			8.00E-05
H	0.03

Yield (mg/mL): 3.85 Dilution Factor: 10×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-08 14:20:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.54E-01	3.71E-01	3.58E-01
B	25.00	2.80E-01	2.85E-01	2.93E-01
C	8.33	2.16E-01	2.18E-01	2.22E-01
D	2.78	1.31E-01	1.27E-01	1.26E-01
E	0.93	5.30E-02	5.37E-02	5.72E-02
F	0.31	1.93E-02	1.98E-02	1.95E-02
G	0.10	6.34E-03	6.73E-03	5.97E-03
H	0.03	2.19E-03	2.23E-03	2.15E-03

Yield (mg/mL): 3.35 Dilution Factor: 100×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-08 14:14:09.

Design Data

Rosetta Score for W399Q: 6.356

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.