D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R246K

Expression Data

Yield (mg/mL): 2.75

Kinetic Assay Data

**slope of R246K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.14E-01	2.19E-01	2.22E-01
B	25.00	1.73E-01	1.69E-01	1.70E-01
C	8.33	1.29E-01	1.29E-01	1.32E-01
D	2.78	7.18E-02	7.29E-02	7.53E-02
E	0.93	2.90E-02	3.01E-02	3.16E-02
F	0.31	1.01E-02	1.07E-02	1.17E-02
G	0.10	3.39E-03	3.48E-03	3.92E-03
H	0.03	1.17E-03	1.17E-03	1.30E-03

Yield (mg/mL): 2.75 Dilution Factor: 100×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-08 14:26:56.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.54E-01	3.71E-01	3.58E-01
B	25.00	2.80E-01	2.85E-01	2.93E-01
C	8.33	2.16E-01	2.18E-01	2.22E-01
D	2.78	1.31E-01	1.27E-01	1.26E-01
E	0.93	5.30E-02	5.37E-02	5.72E-02
F	0.31	1.93E-02	1.98E-02	1.95E-02
G	0.10	6.34E-03	6.73E-03	5.97E-03
H	0.03	2.19E-03	2.23E-03	2.15E-03

Yield (mg/mL): 3.35 Dilution Factor: 100×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-08 14:14:09.

Design Data

Rosetta Score for R246K: -0.377

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.