D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L350I

Expression Data

Yield (mg/mL): 3.41

Kinetic Assay Data

**slope of L350I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.77E-01	2.72E-01
B	25.00	2.35E-01	2.54E-01	2.04E-01
C	8.33	1.77E-01	2.09E-01	1.90E-01
D	2.78	1.18E-01	1.36E-01	1.23E-01
E	0.93	4.22E-02	6.13E-02	4.78E-02
F	0.31	1.60E-02		1.85E-02
G	0.10	5.70E-03		5.20E-03
H	0.03	1.90E-03		2.30E-03

Yield (mg/mL): 3.41 Dilution Factor: 10×

Protein purified on 7/1/2024 and assayed on 7/8/2024.
Data uploaded by HollyTruong and last updated on 2024-07-10 13:19:06.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.39E-01	1.45E-01	1.43E-01
B	25.00	1.37E-01	1.38E-01	1.32E-01
C	8.33	1.38E-01	1.46E-01	1.24E-01
D	2.78	8.32E-02	7.12E-02	9.26E-02
E	0.93	3.45E-02	3.23E-02	3.42E-02
F	0.31	1.48E-02	1.21E-02	1.25E-02
G	0.10	5.90E-03	4.45E-03	3.95E-03
H	0.03	1.50E-04	0	2.00E-04

Yield (mg/mL): 2.268 Dilution Factor: 100×

Protein purified on 6/17/2024 and assayed on 7/18/2024.
Data uploaded by HollyTruong and last updated on 2024-06-24 12:48:40.

Design Data

Rosetta Score for L350I: 0.09

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.