D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N270W

Expression Data

Yield (mg/mL): 2.85

Kinetic Assay Data

**slope of N270W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.46E-01	2.78E-01	2.43E-01
B	25.00	1.77E-01	2.27E-01	2.23E-01
C	8.33	1.59E-01	1.67E-01	1.72E-01
D	2.78	8.87E-02	8.92E-02	8.64E-02
E	0.93	3.91E-02	3.57E-02	3.72E-02
F	0.31	1.31E-02	1.40E-02	1.24E-02
G	0.10	5.26E-03	4.50E-03	4.41E-03
H	0.03	1.86E-03	1.65E-03	1.57E-03

Yield (mg/mL): 2.85 Dilution Factor: 100×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-11 11:54:25.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.82E-01	3.91E-01	3.75E-01
B	25.00	2.71E-01	2.99E-01	3.06E-01
C	8.33	2.21E-01	2.18E-01	2.36E-01
D	2.78	1.24E-01	1.30E-01	1.37E-01
E	0.93		5.66E-02	6.10E-02
F	0.31	1.98E-02	2.01E-02	2.16E-02
G	0.10	6.69E-03	6.73E-03	6.68E-03
H	0.03	2.42E-03	2.37E-03	2.72E-03

Yield (mg/mL): 3.35 Dilution Factor: 100×

Protein purified on 4/22/24 and assayed on 4/24/24.
Data uploaded by mvlouie and last updated on 2024-07-08 15:52:08.

Design Data

Rosetta Score for N270W: 8.535

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.