Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.114	0.120
	B	25.00	0.0790	0.0784
	C	8.33	0.0304	0.0836
	D	2.78	0.0467	0.0489
	E	0.93	0.0407	0.0330
	F	0.31	0.0141	0.0139
	G	0.10	0.00520	0.00360
	H	0.03	0.00460	0.00330

Yield (mg/mL): 0.834     Dilution Factor:  100×

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Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	6.65E-01
	B	31.8	7.93E-01
	C	33.6	7.79E-01
	D	35.5	0.639
	E	37.3	0.724
	F	39.1	0.677
	G	40.9	0.259
	H	42.7	0.0309
	I	44.5	0.0012
	J	46.4	0.0004
	K	48.1	0.0003
	L	50.0	0.0002

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Protein purified on 4/14/21 and assayed on 4/26/21.
Data uploaded by markjo04 and last updated on 2022-07-28 13:47:07.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.