D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F243H

Expression Data

Yield (mg/mL): 4.974

Kinetic Assay Data

**slope of F243H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		5.32E-01	5.47E-01
B	25.00		2.78E-01	3.14E-01
C	8.33	2.30E-01	2.14E-01	2.07E-01
D	2.78	1.74E-01	1.83E-01	1.24E-01
E	0.93	1.75E-01	1.19E-01	1.38E-01
F	0.31	7.81E-02	6.02E-02	8.18E-02
G	0.10	3.67E-02		3.90E-02
H	0.03	7.50E-04	0	2.00E-04

Yield (mg/mL): 4.974 Dilution Factor: 100×

Protein purified on 7/8/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 12:58:52.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.34E-01	3.29E-01
B	25.00	3.25E-01	2.44E-01	2.85E-01
C	8.33	2.46E-01	2.26E-01	2.25E-01
D	2.78	2.11E-01	1.88E-01	1.91E-01
E	0.93	1.76E-01	1.50E-01	1.09E-01
F	0.31	7.10E-02	7.82E-02
G	0.10	2.70E-02	3.99E-02	1.90E-02
H	0.03		0	0

Yield (mg/mL): 5.144 Dilution Factor: 100×

Protein purified on 7/16/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 13:04:14.

Design Data

Rosetta Score for F243H: 2.445

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.