D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H384I

Expression Data

Yield (mg/mL): 2.95

Kinetic Assay Data

**slope of H384I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.56E-01	1.61E-01
B	25.00	1.54E-01	1.37E-01	1.37E-01
C	8.33	1.18E-01	9.71E-02	1.19E-01
D	2.78	9.55E-02	7.84E-02	7.40E-02
E	0.93	4.97E-02	5.07E-02	5.60E-02
F	0.31	2.35E-02	2.45E-02	2.33E-02
G	0.10	1.05E-02	1.27E-02	1.19E-02
H	0.03	2.50E-04	0	1.50E-04

Yield (mg/mL): 2.95 Dilution Factor: 100×

Protein purified on 7/16/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 14:47:41.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.34E-01	3.29E-01
B	25.00	3.25E-01	2.44E-01	2.85E-01
C	8.33	2.46E-01	2.26E-01	2.25E-01
D	2.78	2.11E-01	1.88E-01	1.91E-01
E	0.93	1.76E-01	1.50E-01	1.09E-01
F	0.31	7.10E-02	7.82E-02
G	0.10	2.70E-02	3.99E-02	1.90E-02
H	0.03		0	0

Yield (mg/mL): 5.144 Dilution Factor: 100×

Protein purified on 7/16/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 13:04:14.

Design Data

Rosetta Score for H384I: 12.905

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.